Fig. 4.

ORA59 binds to the PDF1.2 promoter in vivo. Arabidopsis seedlings from XVE-ORA59-TAP line #4 and 35S-TAP line #7 were treated with 4 μM estradiol (E) or 0.1% DMSO (D). RNA and protein was extracted for Northern and Western blot analysis of transgene expression. Sonicated chromatin prepared from the remainder of the tissue samples was used in ChIP with IgG Sepharose which has affinity for the TAP tag. a ChIP analysis. Input chromatin or recovered chromatin preparations were used as templates in PCR with cycle number and gene-specific primers as indicated. b Northern blot analysis with probes as indicated. c Western blot analysis with Peroxidase anti-Peroxidase (PAP) antibodies