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. 2010 Jul 9;9:183. doi: 10.1186/1476-4598-9-183

Figure 4.

Figure 4

The effect of metal ions on XIAP expression. (A) Expression of XIAP in prostate cancer cells was prevented by cadmium only but not by other tested metal ions. PC-3 cells were transfected with the HA-tagged XIAP construct under the control of the SV40 promoter. Four hours after transfection cell culture medium was replaced with medium containing 30 μM of indicated metal salts and cells were cultured for additional 16 hours. Expression of XIAP and α-actin was detected by immunoblotting with anti-HA or anti-actin antibodies respectively. (B) PC-3 cells were transfected with the HA-tagged XIAP construct under the control of the SV40 promoter. Four hours after transfection cell culture medium was replaced with medium containing cadmium (30 μM) and cells were cultured for an additional 16 hours with or without equimolar concentration of zinc. In an additional set of experiments, cells were first incubated in cadmium-containing medium. After 16 hours, all medium containing cadmium was removed and replaced with fresh medium. Cells were then incubated for an additional 4 hours in the absence of cadmium.