γHV68 latency in different subsets of splenic DCs. The frequency of γHV68 genome-positive cells was determined by LDA-PCR assay in FACS-purified splenic DC subsets during the establishment of the latency phase of infection (14 days postinfection, ●) and long-term latency (3 mo postinfection, ○). CD11c+CD8+CD4− (A), CD11c+CD8−CD4+ (B), CD11c+ CD8−CD4− (C), and CD11c+B220+(D).