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. Author manuscript; available in PMC: 2011 Feb 24.
Published in final edited form as: J Immunol. 2005 Sep 1;175(5):3225–3234. doi: 10.4049/jimmunol.175.5.3225

Table I.

γHV68 frequencies within splenic DC (CD11c+) subsets during the establishment and maintenance phases of latent infection

Cells Reciprocal Frequency of Genome-Positive Cellsa % of Total Spleenb Total No. of Cellsc Latently Infected Cellsd Ratioe
14 days postinfection
 B220+ 3,102 1.07 2.14 × 106 690 0.117
 CD8+CD4− 2,963 2.31 4.62 × 106 1559 0.264
 CD8−CD4− 4,769 4.7 9.4 × 106 1971 0.334
 CD8−CD4+ 1,212 1.01 2.02 × 106 1667 0.283
3 mo postinfection
 B220+ 7,328 0.48 2.88 × 105 39 0.419
 CD8+CD4− 74,336 1.23 7.38 × 105 10 0.107
 CD8−CD4− 21,037 1.11 6.66 × 105 32 0.334
 CD8−CD4+ 13,432 0.26 1.56 × 105 12 0.129
a

Frequencies with 95% confidence limits were determined by linear regression analysis of LDA-PCR data. Data are the mean of three independent experiments, each analyzing pooled spleens from five to seven mice.

b

Percentage of each subset of total spleen DC was determined by FACS analysis.

c

Total number of each DC subset per spleen based on estimate of 2 × 108 total cells/spleen at 14 days postinfection and 6 × 107 cells/spleen at 3 mo postinfection.

d

Number of latently infected cells based on the frequency of viral genome-positive cells within each cell type and the estimated total number per spleen.

e

The subset:total ratio was determined by dividing the absolute number of infected cells in each DC subset by the total number of infected DC in the spleen at each given time point.