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. Author manuscript; available in PMC: 2011 Feb 24.
Published in final edited form as: J Immunol. 2010 Mar 5;184(7):3850–3856. doi: 10.4049/jimmunol.0902935

FIGURE 5.

FIGURE 5

CD4 T cells degranulate in the absence of IFN-γ. A, IFN-γ was neutralized in long-term (>3 mpi) γHV68-infected mice by in vivo administration of anti–IFN-γ Ab for 1 wk prior to a degranulation assay using isotype-treated γHV68-infected mice as controls. CD4 T cell splenocytes were left unstimulated or stimulated with virus or PMA/ionomycin. Five hours later, surface CD107 and intracellular IFN-γ was measured by FACS. B, IFN-γ neutralization was confirmed by ELISA in the serum of anti–IFN-γ–treated mice relative to mice treated with isotype control Ab. C, Analysis of CD107 degranulation on long-term (>3 mpi) γHV68-infected IFN-γ−/− mice compared with noninfected IFN-γ−/− controls. Error bars represent SD.