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. 2011 Feb 7;108(8):3424–3429. doi: 10.1073/pnas.1015162108

Fig. 3.

Fig. 3.

Auxin regulates leaf margin development via repression of CUC2. (A–F) Silhouettes of fifth rosette leaf are shown for all genotypes. The auxin signaling mutants axr1-3 (B) and bdl/BDL (C) have more serrated leaf margins than wild type (A). axr1-3;cuc2-3 (D) and bdl/BDL;cuc2-3 (E) double mutants mimic the smooth leaf margins of cuc2-3 (F). (G) Quantitative RT-PCR analysis showed that axr1-3 and bdl/BDL plants displayed elevated CUC2 gene expression compared with wild type. (H and I) Confocal micrographs showing CUC2::CUC2:VENUS expression (yellow) in fifth rosette leaf 125 μm in length in wild type (H) and axr1-3 (I). (Scale bars: A–F, 1 cm; H and I, 25 μm.) Error bars represent SE of mean from three biological replicates.