Fig. 3.

Strategy for assessing tertiary folding. (A) Two scenarios. Depicted are predictions for relative pegylation kinetics for individually engineered cysteines in three component segments of the pore loop between S5 and S6 for an unfolded tertiary pore loop (Left) and a reentrant pore loop (Right). (B) Monomeric constructs for the accessibility assay include Kv1.3 T1(-) (Upper), which lacks only the N-terminal residues 1–141, and a Kv1.3 S5-P-S6C-terminus fragment (FRAG; residues 336–523) (Lower).