Fig. 2.

Geminin antagonizes the suppression of neural gene expression by growth factors. GemOE cells were differentiated for 3 d with addition of hBMP4 (5 ng/mL), mWnt3a (10 ng/mL), or Activin A (1 ng/mL) from days 1–3, without or with simultaneous overexpression of Gem (by 500 ng Dox treatment). At day 3, RNA expression levels were determined using qRT-PCR. Y axis of the graphs shows fold changes in neural gene expression (A) with versus without growth factor addition and (B–D) in Dox-treated versus untreated GemOE cells on day 3 of N2B27 differentiation (baseline level without growth factor treatment = 1.0). ***P < 0.001, **P < 0.01, *P < 0.05, ⁺P < 0.1, ns, not significant.