Fig. 4.

Geminin associates with neural genes before transcriptional activity. (A) Chromatin extracted from ES cells differentiated for 2 d was immunoprecipitated with histone H3 or H3K9ac antibodies. Crosslinks were reversed and equal amounts of total proteins (estimated using Biorad protein assay) were immunoblotted with anti-Gem (N18) antibody or anti-histone H4 antibody. (B) Chromatin from ES cells differentiated for 2 d was immunoprecipitated with anti-Gem or control IgG antibodies. DNA retrieved from ChIP was analyzed using gene-specific ChIP primers (Table S3). (C) qChIP for the proteins/histone modifications indicated was performed at the Sox1 and Pax6 genes on days 0, 2, and 3 and represented as % input (of total input material).