Fig. 2.

EF4 distribution between cytosol and membranes. (Left) Immunoblotting using polyclonal anti-EF4 antibody as described in Materials and Methods. Total, the total amount of EF4 in a cell sample corresponding to the cell fraction used for EF4 determination in the cytosol and membranes, respectively. (Center) Estimates of the relative amounts (%) of EF4 in membrane and cytosol fractions based on the intensities of the corresponding immunoblotting bands. (Right) Molar ratio between EF4 and ribosomal protein S7 in the cytosol fraction based on intensities of corresponding immunoblotting bands (14), using polyclonal anti-EF4 and anti-S7 antibodies; band intensities were calculated as described in Materials and Methods.