Fig 4.

Alterations in metabolic pathways observed in cells expressing IDH1-R132H, expressing IDH2-R172K, or treated with 2HG. The fold difference in metabolite in each experiment relative to vector is indicated by the color of each box. (A) Amino acids and N-acetylated amino acids (IDH1-R132H, left boxes; IDH2-R172K, center boxes; 30 mM 2HG, right boxes). (B) Glutathione and metabolites involved in its regeneration. (C) BCAAs and catabolites. (D) Choline, GPC, and intermediates. (E) TCA and shuttling of citrate, isocitrate, and α-ketoglutarate to the cytosol. Dashed lines indicate exchange of a metabolite between the mitochondria and cytosol. cysH-gly, cysteinylglycine; γ-glu-aa, γ-glutamyl amino acids; γ-glu-cys, γ-glutamyl-cysteine.