Fig. 5.

Atoh1 activates and colocalizes with CGP differentiation markers. (A) Colocalization of Pax6, NeuroD6, NeuroD1, Nhlh1/2, and Sema7a (red channel) with Atoh1 (green channel) in 20-μm sagittal sections of P0 and P5 Atoh1^GFP/GFP cerebella. (B) Using Atoh1^CrePR/flox, Rosa^YFP/YFP mice, we depleted Atoh1 at P5 and collected the cerebellum 24 h later. In the 20-μm sagittal sections, Atoh1 depleted cells were visualized by YFP expression (green channel). The differentiation marker Tuj1 (red channel) colocalized with Atoh1-independent cells located in the IGL, but it was absent from Atoh1-dependent cells located in the EGL. Although the expression of Pax6 and NeuroD6 is Atoh1-independent, the expression of NeuroD1, Nhlh1/2, and Sema7a is Atoh1-dependent while the CGPs still reside in the EGL. Colocalization is shown in white. (Scale bars, 20 μm.)