Fig. 1.

HFD-induced severe obesity in SMRT^mRID1 mice. (A) Generation of SMRT^mRID1 mutant knock-in mice. (B) Bodyweight curves in WT and SMRT^mRID1 on normal chow or HFD. (C) WAT accumulation in 14-wk HFD-treated WT and SMRT^mRID1 mice. (D) Body weight composition of WT and SMRT^mRID1 mice. (E) Wet weight of epidydimal (Epi), mesenteric (Mes), inguinal (Ing), subcutaneous (Sub), and brown fat (BAT) depots in WT and SMRT RID1 mice. (F and G) Hematoxylin and eosin staining of histological sections and adipocyte cross-sectional area from WAT depots in WT and SMRT^mRID1 mice. (Scale bar, 50 μm.) (H) Expression of inflammatory cytokines in WAT from WT and SMRT^mRID1 mice. All error bars are SD. *P < 0.05; **P < 0.01; n.s., not significant unless otherwise indicated.