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. 2011 Feb 7;108(8):3222–3227. doi: 10.1073/pnas.1014975108

Fig. 4.

Fig. 4.

Expression of M1GS RNA (A), viral mRNAs (B), and proteins in vivo (C). Spleens, livers, and lungs were isolated from SCID mice that were intragastrically inoculated with SL101 carrying different constructs and either mock-infected (lanes 1–7, 8, and 12) or infected with MCMV (lanes 9–11 and 13–15), and were harvested at 14 days after infection. Northern and Western analyses were carried out using RNA (A and B) or protein samples (C) isolated from different organs of animals that received SL101 carrying pU6 (-, lanes 1, 8, 9, 12, and 15), pU6-M1-B (lanes 3, 5, 7, 10, and 14), or pU6-M1-A (lanes 2, 4, 6, 11, and 13). The levels of the mouse RNase P RNA (mP1) and actin protein were used as the internal controls. (D) Virulence and toxicity of Salmonella in SCID mice. SCID mice (five animals per group) were infected intragastrically with ST14028 (1 × 103 cfu), SL7207 (5 × 105 cfu), or SL101 (1 × 109 cfu) carrying pU6-M1-A, and their survival was recorded.