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. Author manuscript; available in PMC: 2011 Aug 15.
Published in final edited form as: Dev Biol. 2010 Dec 11;350(2):464–475. doi: 10.1016/j.ydbio.2010.12.010

Figure 3. Hh causes Cdkn1c to stabilize Myod.

Figure 3

(A). Cdkn1c mRNA is Hh-dependent in adaxial cells. Shhatbx392 or smob641 mutation or cyA treatment reduces cdkn1c mRNA. Note that loss was proportional to reduction in Hh signal (arrowheads and black bracket). Shha mRNA injection caused unilateral somitic cdkn1c up-regulation (red bracket). (B,C). Cdkn1c 5′ MO injection into embryos from a shhatbx392/+ incross reduced adaxial myog mRNA (B), Myod or MyHC proteins (C). Note delay in myog mRNA and reduction in Myod protein (brackets). (D). Cdkn1c ATG MO injection reduces adaxial Myod at tailbud stage. Bar: 100 μm.