Table 2. Prevalence of Morogoro virus and Morogoro virus–specific antibodies in Mastomys natalensis mice from Morogoro University campus, Tanzania.

Specimen and year of sampling	No. samples	No. (%) virus positive (PCR)	No. (%) antibody positive*	No. (%) antibody plus virus positive
Serum 2004	96	1 (1)†	42 (44)	0
Liver 2004	303	12 (4)†	–	–
Serum 2007	63	4 (6)‡	40 (63)	3 (5)§

*By immunofluorescent antibody (IFA) assay, performed with Morogoro virus-infected cells (cut-off 32).
†Testing was performed with universal Old World arenavirus large (L) gene reverse transcription–PCR (9).
‡Testing was performed with Morogoro virus–specific L gene RT-PCR using primers MoroL3359-forward (5′-AGGATTAGTGAGAGAGAGAGTAATTC-3′) and MoroL3753-reverse (5′-ACATCATTGGGCCCCACTTACTATGGTC-3′).
§Titers ranged from 64 to 512.