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. Author manuscript; available in PMC: 2011 May 18.
Published in final edited form as: Neuron. 2010 Nov 18;68(4):695–709. doi: 10.1016/j.neuron.2010.09.027

Figure 1. Extension of MADM to Mouse Chromosome 11.

Figure 1

(A) The Hipp11 genomic locus in cytoband A1 (∼3 cM) between Eif4enif1 and Drg1 genes.

(B) Targeting of Hipp11 with GT and TG cassettes to generate MADM-11GT and MADM-11TG. Top panel shows the organization of the Hipp11 genomic locus. Grey boxes indicate exons 1 and 19 of the flanking gene Eif4enif1, and exons 1 and 9 of Drg1. Middle and bottom panels show the Hipp11 genomic locus with integrated GT and TG cassettes. LoxP (black triangles), FRT (rectangle), and the direction of transcription (green arrow) are indicated. Details about recombination products and reconstituted marker genes upon CRE/FLPe-mediated interchromosomal recombination can be found in Figures S1–S3.

(C–H) GFP (green in C–E and H; white in F) and tdT (red in C–E and H; white in G) expression in cortex and hippocampus in P21 MADM-11 mice. (C and H) Sparse MADM labeling of cortical pyramidal cells in MADM-11GT/TG;Rosa26FLPe/+ (C) or MADM-11GT/TG;Nestin-spCre+/− (H). (D) Overview of labeling pattern in MADM-11GT/TG;Emx1Cre/+. (E–G) Higher magnification of D (boxed area) illustrating CA3 pyramidal cells and mossy fiber projections from dentate gyrus granule cells. (I) Schematic depicts TM-mediated MADM-clone induction at E10 in MADM-11GT/TG;Nestin-CreER+/− in symmetrically dividing neuroepithelial stem cell (NESC). A G2-X event (see Figure S1 for a description of the MADM principle) results in two columns of green and red labeled neurons migrating along the processes of radial glia progenitor cells (RGPCs).

(J–M) A G2-X MADM clone in the cortex with neurons expressing GFP (green) and tdT (red), and migrating along RGPCs at E16 (J). Inset in (J) marks area shown in (K)–(M) and depicts red and green apical endfeet of MADM-labeled radial glia with migrating neurons in the VZ. Nuclei (D, J, and K) were labeled using DAPI (blue). CP, cortical plate; IZ, intermediate zone; SVZ, subventricular zone; VZ, ventricular zone; TM, Tamoxifen.

Scale bar, 100 μm (C); 1 mm (D); 30 μm (E–G and K–M); 70 μm (H and J). See also Figures S1 and S2.