Figure 2. MADM Analysis of Lis1, Ndel1, and 14-3-3ε in Somatosensory Cortex.

(A) Genomic location of MADM-11 and Lis1, Ndel1 and 14-3-3ε genes on Chr. 11. Physical and genetic distances to the centromere are indicated.

(B–E) MADM-labeled cells in P21 somatosensory barrel cortex in control-MADM (B; MADM-11^GT/TG;Emx1^Cre/+), Lis1-MADM (C; MADM-11^GT/TG,Lis1;Emx1^Cre/+), Ndel1-MADM (D; MADM-11^GT/TG,Ndel1;Emx1^Cre/+) and 14-3-3ε-MADM (E; MADM-11^{GT/TG, 14-3-3ε};Emx1^Cre/+). In control-MADM (B), GFP⁺ (green), tdT⁺ (red), and GFP⁺/tdT⁺ (yellow) cells are all WT. In Lis1-, Ndel1-, and 14-3-3ε MADM (C–E), homozygous mutants are GFP⁺ (green), heterozygous cells are GFP⁺/tdT⁺ (yellow) or unlabeled (vast majority), and homozygous WT cells are tdT⁺ (red). Nuclei were stained using DAPI (blue). White star in (B) marks tdT⁺ cortical astrocytes. Arrows in (C) indicate sparse green Lis1^−/− mutant neurons. Arrows in (D) point to Ndel1^−/− astrocytes. Cortical layers are numbered in roman digits. WM: white matter. Scale bar, 150 μm.

(F) Quantification of green/red ratio of neurons (upper panel) and cortical astrocytes (lower panel) corresponding to respective genotypes in (B–E). No Lis1^−/− mutant astrocytes were observed in any sample analyzed.

(G–J) Quantification of the relative distribution (%) of mutant green, heterozygote yellow and WT red neurons (upper panels) and astrocytes (lower panels) for genotypes corresponding to (B)–(E). Values represent mean ± SEM ns: nonsignificant, *p < 0.05, **p < 0.01, and ***p < 0.001.

See also Figures S2–S4.