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. Author manuscript; available in PMC: 2011 May 18.
Published in final edited form as: Neuron. 2010 Nov 18;68(4):695–709. doi: 10.1016/j.neuron.2010.09.027

Figure 3. MADM Analysis of Lis1, Ndel1, and 14-3-3ε in the Hippocampus.

Figure 3

(A–D) MADM-labeled cells in P21 CA1 hippocampus in control- (A), Lis1- (B), Ndel1- (C), and 14-3-3ε- (D) MADM. Genotypes and fluorescent labeling are as depicted in Figure 2. The CA1 layer is indicated in (A) and double arrow indicates the distance of the CA1 field from the base of the hippocampus. White arrows in (B) mark ectopic CA1 cell masses. Green stars mark ectopic cells in (C) and (D). Scale bar, 50 μm.

(E) Relative distribution (%) of pyramidal cells within the CA1 layer (CA1) or at ectopic locations (ect. CA1) in control- (top), Ndel1- (middle), and 14-3-3ε- (bottom) MADM.

(F) Distance (μm) of CA1 pyramidal cell soma from the base of the hippocampus in CA1 in control-, Lis1-, Ndel1-, and 14-3-3ε-MADM. Values represent mean ± SEM ns: nonsignificant; **p < 0.01 and ***p < 0.001.

(G) Schematic summary of migration of WT (red) and Ndel1−/− (green) MADM-labeled hippocampal CA1 and CA3 pyramidal neurons (CA) and dGCs. Red WT CA1 and CA3 pyramidal neurons migrate radially and exit the ventricular zone to form the pyramidal cell layers (gray circles). Green Ndel1−/− CA1 and CA3 pyramidal neurons remain at the base of the CA1/CA3 subfields. Red WT dGCs migrate radially to different sublayers of the dentate gyrus granule layer (gray circles). Green Ndel1−/− dGCs accumulate at the hilus and at the base of the dentate granule cell layer but do not properly invade the target layer.

See also Figure S5.