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. Author manuscript; available in PMC: 2011 May 18.
Published in final edited form as: Neuron. 2010 Nov 18;68(4):695–709. doi: 10.1016/j.neuron.2010.09.027

Figure 6. Ndel1 Cell Autonomously Regulates Cortical Neuron Migration into the Cortical Plate.

Figure 6

(A–H) Time course analysis of migration pattern of MADM-labeled cortical projection neurons using in control- (A, C, E, and G) and Ndel1-MADM (B, D, F, and H) at E12 (A and B), E14 (C and D), E16 (E and F) and P1 (G and H). Genotypes are indicated, Ndel1−/− cells labeled with GFP (green) and WT cells with tdT (red).

(I–P) Clonal analysis in MADM-11GT/TG,Ndel1;Nestin-CreER+/− embryonic cortex. (I–L) TM was applied at E10 (TM/E10) and sample analyzed at E16 (A/E16). G2-X clone is illustrated with Ndel1+/+ cells (red in I and L; white in J) and Ndel1−/− cells (green in I and L; white in K). White star marks the border of the cortical plate where Ndel1−/− cells accumulate. (M–P) Time course of clonal analysis in MADM-11GT/TG,Ndel1;Nestin-CreER+/− with TM applied at E8 (M) or E10 (N–P) and samples analyzed at E14 (M and N), E16 (O) and E18 (P). Scale bar, 30 μm (A and B); 60 μm (C and D); 100 μm (E, F, I–L, O, and P); 150 μm (G and H); 50 μm (M and N).

(Q–U) Quantification of ratio of green/red cells (Q) and relative distribution (%) of red and green cells in the VZ/SVZ, IZ, and CP (R–U). Genotypes (top) indicate control-MADM (left column) and Ndel1-MADM (right column) using Emx1Cre/+ at E14 (R), E16 (S), and P1 (T) or Nestin-CreER+/− (TM/E10; A/E16) (U). Values represent mean ± SEM ns, nonsignificant; *p < 0.05 and ***p < 0.001.