Table 2.
Primers used in cloning, chromosomal mapping and real-time RT-PCR for porcine Dab1 and Fyn isoforms
| Primers | Application | Size(bp) | Sequence (5' to 3') |
|---|---|---|---|
| P1 | Porcine Dab1 cloning | 1867/1750 | 5'-ATGTCAACTGAGACAGAACTTCAAGTAGC-3' |
| P2 | 5'-TCCCTCTTCCAAGCGAGTTCC-3' | ||
| P3 | Porcine Fyn cloning | 1614/1605 | 5'-ATGGGCTGTGTGCAATGTAAGGA-3' |
| P4 | 5'-TTACAGGTTTTCACCGGGTTGATAC-3' | ||
| P5 | sDab1 mapping | 321 | 5'-ATGACCGACTGGGCTTACTGTTC-3' |
| P6 | 5'-CCAAATCACACAGCTTGAAAGAGTC-3' | ||
| P7 | sFyn mapping | 261 | 5'-CTGCCTCAGATTTCCAGACGC-3' |
| P8 | 5'-AGCAGGATGAGGTCAGGAGCC-3' | ||
| P9 | sDab1 expression | 207 | 5'-CCATCCAAATCATCTGCATCCC-3' |
| P10 | 5'-GACCTGTGCTATCTAGCTACCGGC-3' | ||
| P11 | sDab1-Li expression | 176 | 5'-GTATCAGGTTCCCACCAGCCAA-3' |
| P12 | 5'-GGGTCACAGCCTTTGTTGCCT-3' | ||
| P13 | sFyn-B expression | 179 | 5'-AAAGGGATGCCAAGGCTTACC-3' |
| P14 | 5'-GACATTGTGCCTGGTTTGAGAGTC-3' | ||
| P15 | sFyn-T expression | 186 | 5'-AACTGTGATTGCATCGAGTTGTACC-3' |
| P14 | 5'-GACATTGTGCCTGGTTTGAGAGTC-3' | ||
| P17 | GAPDH expression | 220 | 5'-GCAAATTCCACGGCACAGTCA-3' |
| P18 | 5'-TCAGCAGAAGGGGCAGAGATG-3' |