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. 2011 Feb 3;12:96. doi: 10.1186/1471-2164-12-96

Table 1.

Primer sequences used in quantitative qRT-PCR analyses

Gene name Gene symbol Sense primer
(5'-3')
Antisense primer
(5'-3')
3-hydroxy-3-methylglutaryl-Coenzyme A reductase Hmgcr1 cttgtggaatgccttgtgattg agccgaagcagcacatgat
Low density lipoprotein receptor Ldlr1 aggctgtgggctccatagg tgcggtccagggtcatct
Insulin induced gene 1 Insig1 2 tcacagtgactgagcttcagca tcatcttcatcacacccaggac
Peroxisome proliferator activated receptor α Ppara 3 cctcttcccaaagctccttca cgtcggactcggtcttcttg
Deiodinase iodothyronine type II Dio24 Mm00515664_m1
Interleukin-6 Il64 Mm00446190_m1
Glucose-6 phosphatase G6Pc4 Mm00839363_m1
18 S ribosomal RNA 18S5 cgccgctagaggtgaaattc ttggcaaatgctttcgctc
β-actin Actb ccgtgaaaagatgacccagatc cacagcctggatggctacgt

1 Reference [41]; 2 Reference [42]; 3 Reference [43] ; 4 Assay-on-demand (Applied Biosystems) 5 Reference [44]