Table 1.

Intra-day accuracy and intra-day precision of imatinib quantification in human blood, leukemia cells and cell culture media

		Human whole blood	Human leukemia cells	Cell culture media
Intra-day accuracy	LLOQ	100.9%	98.1%	93.2%
	0.1 ng/mL	108.6%	100.1%	100.1%
	1 ng/mL	94.7%	100.0%	100.2%
	10 ng/mL	107.8%	107.6%	98.8%
	ULOQ	92.8%	86.5%	94.24%
Intra-day precision	LLOQ	8.8%	13.2%	14.9%
	0.1 ng/mL	7.2%	6.8%	6.8%
	1 ng/mL	6.7%	3.1%	7.3%
	10 ng/mL	8.2%	7.0%	2.4%
	ULOQ	4.3%	5.4%	11.0%
In-process stability	LLOQ	114.1%	118.1%	107.4%
	0.1 ng/mL	97.3%	114.0%	113.3%
	1 ng/mL	93.1%	111.0%	113.7%
	10 ng/mL	102.0%	109.7%	115.9%
	ULOQ	99.6%	112.0%	105.4%

LLOQ, lower limit of quantitation; ULOQ, upper limit of quantitation. LLOQ for human whole blood and leukemia cells: 0.03 ng/mL and for cell culture media 0.01 ng/mL; in-process stability measured after 24 h at +4°C; ULOQ, 75 ng/mL.