Table 1. Bacterial strains and oligonucleotides used in this study.

Strain	Genotype	GFP+	FimH	PapG	LPS	Reference
LT004	CFT073 cobS::Φ(PLtetO-1-gfp+), cmr	X	x	x	Smooth	[19]
UPEC76	CFT073 Δ papDEFG (pheV)Δ papEFG (pheU)	-	x	-	Smooth	[10]
W3110	E. coli K-12 Lab strain	-	x	-	Rough	[55]
ARD40	LT004 fimH::kmr	X	-	x	Smooth	This work
ARD41	UPEC76 cobS::Φ(PLtetO-1-gfp+), cmr	X	x	-	Smooth	This work
ARD42	W3110 cobS::Φ(PLtetO-1-gfp+), cmr	X	x	-	Rough	This work
ARD43	ARD42 pKTH3020	X	x	x	Rough	This work
MC1	Salmonella enterica serovar typhimurium					[56]
Plasmid
pKTH3020	pap operon from KS71			x		[57]
pKD4	Km resistance cassette source					[54]
pKM001	PLtetO-1-GFP+ source plasmid					[19]
Oligos
FimH KO Fa	ATTACCCAGAAACCATTACAGACTATGTCACACTGCAACGTGTGTAGGCTGGAGCTGCTTC
FimH KO Ra	CATCATTATTGGCGTAAATATTCCACACAAACTGGAAATCCATATGAATATCCTCCTTAG
FimA F	GCTCTGTCCCTCAGTTCCAC
FimA R	TCAACAGAGCCTGCATCAAC
PapA F	TGAAATTCGCAACTGCTGAG
PapA R	AACGGGTGAAATTTGATGGA
GFP+ F	ATCCGTTCAACTAGCAGACC
GFP+ R	GTTACAAACTCAAGAAGGACCA

^a40 bp homology to fimH, followed by 20 bp homology to pKD4 (italics).