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. 2011 Feb 24;7(2):e1001300. doi: 10.1371/journal.ppat.1001300

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Total HIV DNA was measured by RU5 quantitative PCR in PBMC from 2 non-controllers off and 4 on HAART and elite suppressor patients. Serial two-fold dilutions (from 1e6 to 2.5e5 cells per well) were measured in triplicates for each sample. This was repeated on a separate extraction for all ES. In all cases inhibitors were demonstrated to be absent by dose response in 3 serial two-fold dilutions. Data from 6 off and 17 on HAART patients previously measured was also included for analysis [21], [22]. Patients off HAART have higher levels of total HIV DNA than other patient populations. The level of total HIV DNA was not significantly different between patients on HAART and ES, while both were significantly lower than patients off HAART (p = 0.002 for both). The line represents the median value.