Figure 4. Effect of glycan content on the α4β7-reactivity.
A) α4β7-reactivity of the ancestral AN1 gp120 (subtype B) produced in CHO-S cells, 293F cells, CHO lec1 cells and kifunensine treated 293F cells, were compared and reported as mean fluorescence intensity (MFI). AN1 V1/V2 PNGΔs were also included. Results are representative of three independent experiments using independent donor CD4+ T cells. B) Positions of all PNGs and the α4β7 binding site in the V1/V2 loop of AN1 gp120 are illustrated.