Table 1. PAX6 PCR primers used in this study.
Primer | Forward (5'-3') | Reverse (5'-3') | Tm (°C) | Product size (bp) |
---|---|---|---|---|
exon1 |
AGGGAACCGTGGCTCGGC |
GGGTGAGGGAAGTGGCTGC |
62 |
207 |
exon2 |
TTATCTCTCACTCTCCAGCC |
GGAGACCTGTCTGAATATTGC |
54 |
307 |
exon3 |
TCAGAGAGCCCATGGACGTAT |
CTGTTTGTGGGTTTTGAGCC |
58 |
193 |
exon4 |
AGTTCAGGCCTACCTGATGC |
GTCGCGAGTCCCTGTGTC |
58 |
201 |
exon5 |
CTCCCTCATCTTCCTCTTCC |
GGGGTCCATAATTAGCATCG |
58 |
327 |
exon6-7 |
GGGCTACAAATGTAATTTTAAGAAA |
AGAGAGGGTGGGAGGAGGTA |
56 |
509 |
exon8 |
GAGCTGAGATGGGTGACTG |
GAGAGTAGGGGACAGGCAAA |
58 |
300 |
exon9 |
AGACTACACCAGGCCCCTTT |
TGAAGATGTGGCATTTACTTTGA |
58 |
291 |
exon10 |
GGAACCAGTTTGATGCACAG |
ACTCTGTACAAGCACCTCTGTCTC |
58 |
243 |
exon11 |
GGGCTCGACGTAGACACAGT |
GGAAACTGAGGGCAAGAGAA |
56 |
300 |
exon12 |
CGGGCTCTGACTCTCACTCT |
GCCACTCCTCACTTCTCTGG |
60 |
220 |
exon13 |
GCTGTGGCTGTGTGATGTGT |
AGGAGATTCTGTTTGGGTA |
52 |
281 |
exon14 | TCCATGTCTGTTTCTCAAAGG | TCAACTGTTGTGTCCCCATAG | 56 | 219 |