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. 2011 Feb 19;17:548–557.

Table 1. PAX6 PCR primers used in this study.

Primer Forward (5'-3') Reverse (5'-3') Tm (°C) Product size (bp)
exon1
AGGGAACCGTGGCTCGGC
GGGTGAGGGAAGTGGCTGC
62
207
exon2
TTATCTCTCACTCTCCAGCC
GGAGACCTGTCTGAATATTGC
54
307
exon3
TCAGAGAGCCCATGGACGTAT
CTGTTTGTGGGTTTTGAGCC
58
193
exon4
AGTTCAGGCCTACCTGATGC
GTCGCGAGTCCCTGTGTC
58
201
exon5
CTCCCTCATCTTCCTCTTCC
GGGGTCCATAATTAGCATCG
58
327
exon6-7
GGGCTACAAATGTAATTTTAAGAAA
AGAGAGGGTGGGAGGAGGTA
56
509
exon8
GAGCTGAGATGGGTGACTG
GAGAGTAGGGGACAGGCAAA
58
300
exon9
AGACTACACCAGGCCCCTTT
TGAAGATGTGGCATTTACTTTGA
58
291
exon10
GGAACCAGTTTGATGCACAG
ACTCTGTACAAGCACCTCTGTCTC
58
243
exon11
GGGCTCGACGTAGACACAGT
GGAAACTGAGGGCAAGAGAA
56
300
exon12
CGGGCTCTGACTCTCACTCT
GCCACTCCTCACTTCTCTGG
60
220
exon13
GCTGTGGCTGTGTGATGTGT
AGGAGATTCTGTTTGGGTA
52
281
exon14 TCCATGTCTGTTTCTCAAAGG TCAACTGTTGTGTCCCCATAG 56 219