Table 2. Primers sequences and the physical position used in real-time quantitative PCR and the results of MLPA and real-time PCR.
| |
|
|
Family 85 |
Family 86 |
||
|---|---|---|---|---|---|---|
| Gene/SNP | Physical position | Primer sequences (5′-3′) | MLPA | RQ result* | MLPA | RQ result* |
|
rs11031332 |
31235538 |
Fwd: CATTAGATTCATGTGGCTTGTTG |
|
1.17 |
||
| |
|
Rev: AATCCGATTCCTCGATTTGT |
|
|
|
|
|
rs1929020 |
31237204 |
Fwd: TGACTGGTGCTACTGCCAAC |
|
|
|
0.49 |
| |
|
Rev: CTCCCGAATAGCGTGGATTA |
|
|
|
|
|
DCDC1 |
31240747–31347897 |
|
- |
|
+ |
|
|
ELP4 |
31487873–31761905 |
|
- |
|
+ |
|
|
rs35674517 |
31763272–31763273 |
Fwd: TTCTTCCCCATTTTCTTTTCAA |
|
1.18 |
|
|
| |
|
Rev: TTCAGAATGTTCATAATGCTTTCAA |
|
|
|
|
|
rs608293 |
31764856 |
Fwd: GAATATTTTTCCCCCAAAGC |
|
0.44 |
|
1.07 |
| |
|
Rev: TGAGAGGCCCAGAGTAAAAAGA |
|
|
|
|
|
PAX6 |
31762916–31796085 |
|
+ |
|
- |
|
|
DKFZp686K1684 |
31794690–31865163 |
|
+ |
|
- |
|
|
RCN1 |
32074656–32083376 |
|
+ |
|
- |
|
| SHGC-143238 |
32102437–32102880 |
Fwd: TCCTCCCCTTGAGTCACTTACAT |
|
0.5 |
|
|
| |
|
Rev: TTACTTGCCAAAACACACTCCCT |
|
|
|
|
|
rs7104670 |
32155485 |
Fwd: GTTACCACATTCGCAAAGCA |
|
0.46 |
|
|
| |
|
Rev: CAGACAAACACATTGACAGTCCT |
|
|
|
|
|
rs12577592 |
32165037 |
Fwd: CCTTGCTCCTTTCAGTCCAG |
|
0.35 |
|
|
| |
|
Rev: ACCCTGAGTGTGTGTCATGC |
|
|
|
|
|
rs4244480 |
32172819 |
Fwd: ACTTATCCCGCCCTTGTGTT |
|
0.41 |
|
|
| |
|
Rev: CACCAGGTTGTTTTGGGACT |
|
|
|
|
|
rs72320219 |
32173790–32173791 |
Fwd: AGGAAAATCGCTTGAACCTG |
|
1.08 |
|
|
| |
|
Rev: GGGGTGGTTGTGAGGACTAA |
|
|
|
|
|
GAPDH |
6516714–6517797 |
Fwd: ACCCAGAAGACTGTGGATGG |
|
|
|
|
| Rev: TTCTAGACGGCAGGTCAGGT | ||||||
Abbreviations: MLPA, Multiplex ligation-dependent probe amplification; RQ, relative quantity; +, deletion ; -, no deletion; *mean value of three times; Fwd, forward; Rev, reverse.