Figure 10. Pathogenicity test of Mossadh and Moact mutant strains on the wounded rice leaves.

(A) Pathogenicity test of Mossadh mutants on the wounded rice leaves. The mycelia blocks of the wild type strain Guy11, Mossadh mutants, and the complemented strain were inoculated on the wounded rice leaves and then cultured under moist conditions with 28°C for 5 days. The wounded rice leaves with the CM agar plugs on was used as negative control. This experiment was performed three times with 10 pieces of rice leaves for each strain. Similar results were obtained in each test and this picture showed the representative result. (B) Pathogenicity test of Moact mutants on the wounded rice leaves. The mycelia blocks of Guy11, Moact mutants and the complemented strain were inoculated on the wounded rice leaves and observations made as above. (C) Pathogenicity test of the mutant strain by injection of hyphae fragments. The hyphae fragments of Guy11, the mutant strains and respective complemented strains were prepared as described in Materials and Methods. From left to right, leaves injected with water, wild type strain, Mossadh mutant, and Moact mutant. The arrowheads in black indicate injection sites with necrosis, while the arrowheads in white indicate injection sites without necrosis.