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Figure A shows primary keratinocytes, Figure B immortalized HaCaT cells. CXCL8, TSLP and IL-6 secretion was measured in triplicates in supernatants after 48 h in medium or chitin-treated cells using ELISA. Chitin fragments were incubated with cells for 48 hours at three different concentrations (C 500 = 0.22 mg/ml; C 1000 = 0.5 mg/ml and C 2000 = 2.0 mg/ml). * p<0.05 of medium compared to chitin treated cells.
