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. 2011 Feb 24;6(2):e16546. doi: 10.1371/journal.pone.0016546

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Li et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The GST-fused PB1 mutants (A, B) or GST-fused PB2 (C, D) mutants were generated. The interaction between GST-PB1 mutants (B) or GST-PB2 mutants (D) and His-Hsp70 was analysed by GST pull-down assay and Western blot (upper panel), the CBB staining of purified proteins were shown in lower panel. (A, C) Binding activity is indicated as “+”, no significant binding activity as “−”. (E) The schematic map of Hsp70 and its mutants. (F) His-Hsp70, His-Hsp70ΔEEVD, His-Hsp70 ATPase domain and His-Hsp70 PBD were prepared and subjected to GST pull-down assays with GST-PB1(79–659) (upper left panel), GST-PB2(1–521) (upper right panel) and GST as negative control, and the purified His-Hsp70 and its mutants were subjected to CBB staining (lower panel). Arrows indicated purified proteins of CBB staining. These experiments were repeated twice with identical results.