Figure 7. Hsp70 inhibits both transcription and replication of RNPs.

HeLa cells were transfected with Hsp70 siRNA and control siRNA for 60 h and infected with rWSN in the presence of 100 µg/ml of CHX for 1 h then cultured with DMEM containing CHX for 7 h, or in the absence of CHX as control. Total cell lysates were prepared for immunoblotting with anti-Hsp70 and anti-β-actin antibodies (A). Total RNA was isolated and subjected to real-time RT-PCR to measure the amounts of mRNA of NP and NS segments. The white bars of control siRNA-transfected cells are set to a relative value of 1, and the black bars represent the relative value campared with control (B). The data represent the means of three independent experiments, each performed in triplicate. Error bars represent standard deviation (n = 3).