Table 8. Comparison of iron-sulfur cluster assembly machineries in organisms with mitosomes (Giardia intestinalis, Cryptosporidium parvum, and Encephalitozoon cuniculi), hydrogenosomes (Trichomonas vaginalis), and mitochondria (Trypanosoma brucei, Saccharomyces cerevisiae).

Name	G. intestinalis	C. parvum	E. cuniculi	T. vaginalis	T. brucei	S. cerevisiae
IscS (Nfs)	•	•	•	••	•	•
Isd11	○	○	•	••	•••	•
Nfu	•	○	○	•••	•••	•
IscU (Isu)	•	•	•	•	•	••
IscA1(Isa1)	○	○	○	○	•	•
IscA2 (Isa2)	•	○	○	••••	•	•
Iba57	○	○	○	○	•	•
Ind	○	○	○	•••	•	•
Grx5	•	○	•	○	•	•
Ferredoxin (Yah1)	•	•	•	•••••••	••	•
FOR (Arh1)	○	•	•	○	•	•
Frataxin (Yfh1)	○	•	•	••	•	•
HSP70	•	•	•	•••	••	•♦♦
Dna-J (Jac1)	•	•	•	••	•	•
GrpE	•	•	•	••	•	•
Atm1	○	•	•	○	•	•
Erv1	○	○	•	○	•	•

Filled circles indicate the presence of protein exhibiting homology to the known component of mitochondrial iron-sulfur cluster assembly machinery identified by BLAST searches. Empty circles indicates absence of homologous protein. Mitochondria of S. cerevisiae possess three distinct Hsp70 of which Ssq1 is devoted for FeS cluster assembly (filled circle), while Ssc1, and Ecm10 have other fuctions (diamonds). Other eukaryotes possess multifunctional Hsp70. IscS, cysteine desulfurase; Isd11, IscS binding protein; Nfu, IscU, IscA, a scafold proteins; Iba57, IscA binding protein required for [4Fe4S] cluster assembly; Ind, P-loop NTPaseb required for assembly of respiratory complex I; Grx5, glutaredoxin 5; ferredoxin, [2Fe2S] ferredoxin that transport electrons; FOR, ferredoxin oxidoreductase; frataxin, iron binding protein; Hsp70, chaperone; DnaJ, GrpE, co-chaperones; Atm1, ABC half trasnporter; Erv1, sulfhydryl oxidase. Names of proteins used for S. cerevisiae orthologs are in brackets.