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. 2011 Feb 24;6(2):e17048. doi: 10.1371/journal.pone.0017048

Figure 2. TGF-β1 induces nuclear translocation of PINCH1 in human podocytes.

Figure 2

A, Western blot analyses show marked induction of PINCH1 protein in the nuclei of podocytes after TGF-β1 treatment. Podocytes were incubated with TGF-β1 at various concentrations as indicated for 72 h. Nuclear and cytoplasmic preparations were made and immunoblotted with specific antibodies against PINCH1, TBP and GAPDH, respectively. B, Graphic presentation shows the relative ratio of the nuclear/cytoplasmic PINCH1 protein in podocytes after TGF-β1 treatment. The value of the nuclear/cytoplasmic ratio of PINCH1 in the control group was set as 1.0. Data are presented as mean ± SEM of four experiments. *P<0.05, **P<0.01 versus controls.