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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: J Neuropathol Exp Neurol. 2011 Jan;70(1):51–62. doi: 10.1097/NEN.0b013e3182032d37

Figure 2.

Figure 2

Figure 2

Figure 2

Microglia in the mouse optic nerve at 6 weeks of age and in mouse optic gliomas at 3 months of age are mainly resident microglia. (A) Flow cytometry demonstrates that the majority of cells from 6-week-old wild type (WT) optic nerves (10 pooled optic nerves) are doubly positive for both CD11b and CD68. (B) The majority of these CD11b+ cells express low levels of CD45 (R1). (C) CD11b+ microglia in the 6-week-old optic nerve are also Iba1+. (D) Using double-labeling immunofluorescence, there are rare cells in the 6-week-old WT optic nerve that label with CD11b antibodies (green) alone (inset, arrowhead), whereas the vast majority (∼99%) of the microglia co-label with both CD11b and Iba1 antibodies (inset, arrows) (n = 5). (E) Iba1+ cells express low levels of CD45 (R1). (F) CD11b magnetic cell capture of microglia from 3-month-old Nf1+/-GFAPCKO mouse optic gliomas (7 pooled optic nerves), followed by flow cytometry demonstrates that the CD11b+ cells are also almost exclusively CD45-low (R1).