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. Author manuscript; available in PMC: 2012 Jan 1.

Published in final edited form as: J Neuropathol Exp Neurol. 2011 Jan;70(1):51–62. doi: 10.1097/NEN.0b013e3182032d37

Reduced CX3CR1 expression in the Nf1^+/- optic nerve (ON). (A) Real-time qPCR reveals no change in Cx3cl1 mRNA levels in ONs from Nf1^+/- (n = 5) (black bars) and wild type (WT) (n = 5) (gray bars) mice at 3 weeks (p = 0.1558), 6 weeks (p = 0.3478), or 3 months (p = 0.4788) of age. (B) qPCR reveals a >2-fold decrease in Cx3cr1 mRNA expression in the ONs of 6-week-old Nf1^+/- mice (n = 5) vs. WT controls (n = 5) (p = 0.0181). There is no difference in Cx3cr1 expression in the ONs from Nf1^+/- vs. WT mice at 3 weeks (p = 0.0855) or 3 months (p = 0.1413) of age. (C) There is a 2-fold increase in numbers of Iba1-positive microglia in ONs of NF^+/+Cx3cr1⁺/GFP mice (n = 6) vs. WT controls (n = 5) (p = 0.0042). Nf1^+/+-Cx3cr1+/GFP mice (n = 7) have greater numbers of microglia vs. WT (p < 0.0001) and Nf1^+/- mice (n = 5) (p < 0.0001).

Reduced CX3CR1 expression in the Nf1^+/- optic nerve (ON). (A) Real-time qPCR reveals no change in Cx3cl1 mRNA levels in ONs from Nf1^+/- (n = 5) (black bars) and wild type (WT) (n = 5) (gray bars) mice at 3 weeks (p = 0.1558), 6 weeks (p = 0.3478), or 3 months (p = 0.4788) of age. (B) qPCR reveals a >2-fold decrease in Cx3cr1 mRNA expression in the ONs of 6-week-old Nf1^+/- mice (n = 5) vs. WT controls (n = 5) (p = 0.0181). There is no difference in Cx3cr1 expression in the ONs from Nf1^+/- vs. WT mice at 3 weeks (p = 0.0855) or 3 months (p = 0.1413) of age. (C) There is a 2-fold increase in numbers of Iba1-positive microglia in ONs of NF^+/+Cx3cr1⁺/GFP mice (n = 6) vs. WT controls (n = 5) (p = 0.0042). Nf1^+/+-Cx3cr1+/GFP mice (n = 7) have greater numbers of microglia vs. WT (p < 0.0001) and Nf1^+/- mice (n = 5) (p < 0.0001).