Fig. 5.

Expression of ataxin-3 mRNA in brain samples from transgenic animals. The 677-bp RT–PCR product, which represents mRNA containing mouse exon 1 and human exons of ataxin-3, was initially not detected in the mut/mut and wt/mut animals using the standard 28 PCR cycles (H). The RT–PCR product that was specific for mouse mRNA containing exon 1 was detected in transgenic animals (mut/mut and wt/mut). The product was shorter by the length of exons 2 and 3 (lines) and was 613 bp rather than the predicted 823-bp wt product, suggesting the alternative splicing event. H human ataxin-3 specific PCR; M mouse ataxin-3 specific PCR