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. 2011 Jan;11(1-2):72–78. doi: 10.1016/j.gep.2010.09.006

Fig. 2.

Fig. 2

Embryonic dPlod expression. A-F, RNA in situ hybridisation (left panel) and dPlod antibody staining (right panel) in Drosophila embryos. (A) Cellular blastoderm, showing that dPlod is not found in the early embryo. (B) Stage 13 embryo, dPlod is detected at high levels in haemocytes throughout the embryo. Haemocytes in the head (black arrowheads) and adjacent to the CNS (white arrowheads) are indicated. (C) Ventral view of a stage 15 embryo showing dPlod expressing haemocytes outlining the CNS. (D) lateral view of a stage 15 embryo showing high levels of dPlod expression in the fat body (arrowheads). (E) Dorsal view of a stage 15 embryo showing dPlod expression in the fat body (arrowheads). (F) dorsal view of stage 17 embryos. (G) Higher magnification of embryo shown in B. (H) High magnification view of haemocytes surrounding the gut in a stage 17 embryo (arrowheads). (I) High magnification view of a stage 16 embryo showing dPlod protein expression in the anal pad. J and K, Stage 16 sibling control (J) or homozygous dPlod deficient (K) embryos stained for dPlod (red) and GFP (green). Homozygous dPlod deficient embryos were identified by absence of CtB-GFP. (L) and (M), p{GAWB}NP5633 GAL4 insertion line crossed to UAS-nuclearLacZ. Stage 16 embryos showing expression in fat body (yellow arrowhead), haemocytes (magenta arrowhead) and anal pads (asterisk). Lateral (L) and ventral (M) views are shown). In all figures anterior is to the left.