Subcellular localisation of dPlod. (A) and (B) dPlod localises to the ER. dPlod (red) in the fat body (A) and haemocytes (B) is coincident with an ER marker (EYFP-ER, green). (C) In contrast there is no overlap between dPlod (green) and a Golgi apparatus marker (EYFP-Golgi, red, overlap would appear yellow. Image from fat body shown; two individual fat body cells are marked with asterisks). (D) dPlod (magenta) expression in haemocytes (srp-Gal4, crq-Gal4, UAS-GFP; green) from a stage 16 embryo. dPlod is detected within haemocytes and extracellular puncta in areas surrounding haemocytes (arrowheads).