Figure 5.

A decrease in glucose concentration inhibits oligodendrocyte development and myelination in cultured cortical brain slices. A, Images of the cerebral cortex in cultured brain slices from mice in which oligodendrocyte lineage cells express GFP under control of the Sox10 promoter. The slices were cultured in solution containing (from left to right) 41.5, 10.5, 5.5, 2.9, and 1.4 mm glucose and immunolabeled for MBP (red) and NF (blue). Sox10-GFP is green. B–D, Normalized fluorescence intensity (see Materials and Methods) for MBP (B), NF (C), and Sox10-GFP (D), pooled from four separate experiments, using a total of 22, 10, 23, 24, and 9 slices for 41.5, 10.5, 5.5, 2.9, and 1.4 mm glucose, respectively. E, F, Labeling intensity ratio for MBP/NF (E) and MBP/Sox10-GFP (F) in 41.5, 10.5, 5.5, 2.9, and 1.4 mm glucose. G, Sox10-GFP fluorescence (arbitrary units) versus number of Sox10-GFP cells (counted using ImageJ), demonstrating a linear relationship between the two in 30 slices cultured in 1.4–41.5 mm glucose (each point is one slice). Error bars indicate SEM.