Primary neurons of the indicated genotype were immunostained with anti-Tom20 (red) and anti-Tubulin III (green) antibodies. Randomly selected confocal, z axis stacks were acquired, stored, reconstructed and volume rendered. Scale bar: 20 µm.
3× magnification of the boxed areas in (A). The bottom panel was turned 90° counter-clockwise.
Morphometric analysis of mitochondrial shape. Experiments were done as in (A). Data represent mean ± SE of three independent experiments (n = 70 stacks).
Representative electron micrographs of wt and YAC128 primary striatal neurons. Cells were fixed and TEM images of randomly selected fields were acquired. Boxed areas are magnified 2.4×. Scale bar: 1 µm.
Cells of the indicated genotype were electroporated with mtRFP and empty vector or the indicated plasmids. Samples were then immunostained with anti-Tubulin III antibody (magenta) and incubated with TUNEL reagent. Images were acquired exactly as in (A). Scale bar: 20 µm.
Morphometric analysis of mitochondrial morphology. Experiments were performed as in (E). Data represent mean ± SE of three independent experiments (n = 40 stacks).
