Figure 6. Increased susceptibility to apoptosis of striatal YAC128 primary neurons: correction by Opa1 and dominant-negative Drp1.

1. Representative confocal images of TUNEL assay of primary striatal neurons. Neurons of the indicated genotype were electroporated with mtRFP, treated where indicated with 500 nM staurosporine for 14 h, immunostained with anti-Tubulin III and processed for TUNEL. Arrowheads, TUNEL positive neurons. Scale bar: 20 µm.
2. Quantification of TUNEL assay of primary neurons. Cells of the indicated genotype were transfected as indicated and treated as in (A). Cell death is represented as the percentage of TUNEL positive cells in the mtRPF–Tubulin III double positive population. Data represent mean ± SE of three independent experiments (n = 300 neurons per condition).