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. Author manuscript; available in PMC: 2011 Feb 25.
Published in final edited form as: Virology. 2006 Jul 14;353(2):247–257. doi: 10.1016/j.virol.2006.06.005

Fig. 2.

Fig. 2

Sustained low magnitude increase in intracellular calcium concentration enhances transcription of p300. (A) Jurkat T cells (1 × 106 per ml) were stimulated with varying concentrations of ionomycin for 36 h. Total cellular RNA was extracted and semi-quantitative RT-PCR was performed to identify the mRNA levels of p300. There was a dose-dependent increase in p300 expression in Jurkat T cells stimulated with low concentrations of ionomycin (25, 50, and 100 nM). (B) Densitometric analysis was performed using alpha imager software and normalized to GAPDH. Dose-dependent increases (up to 2.4-fold) in p300 mRNA were observed in Jurkat T cells stimulated with 100 nM ionomycin. Statistical analysis was performed using Student’s t test, P < 0.05.