Figure 1.

Diphtheria toxin (DT) treatment effectively depletes Foxp3⁺ regulatory T (Treg) cells. Wild-type (WT) C57BL/6 and Foxp3-DTR mice were treated with or without 1 μg diphtheria toxin (DT) on days 0, 1 and 3 relative to onset of experimental autoimmune encephalomyelitis (EAE). Spleen (a) and lymph node (b) were harvested on day 5 after DT treatment and Foxp3 expression was measured by flow cytometry using specific antibodies.