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. 2011 Mar;132(3):340–347. doi: 10.1111/j.1365-2567.2010.03380.x

Figure 4.

Figure 4

Expression of programmed death 1 ligand 1 (PD-L1), CCR6 and activation markers on splenic B cells. Foxp3-DTR mice were administered three doses of diphtheria toxin (DT) and splenocytes from placebo and oestrogen (E2) -treated mice were analysed by flow cytometry on day 4–5 for expression of (a) PD-L1, CCR6, Gr-1 and (b) B7.1 (CD80) and B7.2 (CD86) on specific cell types. Cells were gated on CD19 and CD11c to determine expression of B7.1 and B7.2. One representative experiment of four is shown with a total of 10 placebo-treated and E2-implanted Foxp3-DTR mice.