Table 1.

Differences in cytokine levels measured by Luminex in lung homogenates (Lung) or bronchoalveolar lavage (BAL) of mice over-expressing human interleukin-4 (hIL-4) or its alternatively spliced variant hIL-4δ2 on day 14 after infection with the indicated adenoviruses

		hIL-4		hIL-4δ2		NULL
Cytokine	Sample	Median pg/ml [1st, 3rd quartile]	n	Median pg/ml [1st, 3rd quartile]	n	Median pg/ml [1st, 3rd quartile]	n
TNF-α	Lung	67 [25, 84]*	13	112 [89, 135]**	13	0 [0, 12]	5
TNF-α	BAL	20 [9, 29]*	14	31 [19, 40]**	14	0 [0, 0]	5
KC (IL-8)	Lung	247 [222, 261]*	3	258 [256, 297]*	3	125 [108, 142]	3
KC (IL-8)	BAL	61 [49, 101]*	6	54 [43, 79]*	6	0 [0, 0]	3
IL-1α	Lung	232 [151, 324]*	12	341 [288, 448]**	13	89 [81, 135]	5
TGF-β	BAL	84 [63, 97]*	14	81 [64, 108]*	14	43 [6, 75]	5
IFN-γ	Lung	0 [0, 43]	13	64 [49, 75]**	13	0 [0, 0]	5
IFN-γ	BAL	0 [0, 9]	14	16 [12, 25]**	14	0 [0, 0]	5
IL-12p40	Lung	68 [63, 68]*	3	131 [88, 158]**	3	13 [7, 20]	3
IL-12p40	BAL	154 [136, 169]*	6	186 [159, 190]**	6	60 [48, 71]	3
MCP-1	Lung	0 [0, 2]	10	8 [1, 17]**	12	0 [0, 0]	9

Single asterisks indicate significant differences (P < 0·05, Mann–Whitney U-test) from the AdV-NULL-infected animals. Double asterisks indicate significant differences from both AdV-NULL-infected animals and from animals infected with the other construct (AdV-IL-4δ2 or AdV-IL-4). Data combined from one to three independent experiments performed by two different technicians, with the total number of animals (n) indicated for each cytokine in each group

TNF-α, tumour necrosis factor-α; TGF-β, transforming growth factor-β; IFN-γ, interferon-γ; MCP-1, monocyte chemoattractant protein 1.