Table 2.

Autoreactivity and expression changes for the significant proteins

	IgG reactivity		IgM reactivity		mRNA GSE8021		mRNA GSE9102
Gene symbol	log2 ratio	P	log2 ratio	P	log2 ratio	P	log2 ratio	P	Gene Name
EGFR	1·73	0·0029	1·30	0·011	−0·04	0·55	−0·33	0·083	Epidermal growth factor receptor
MBP	0·93	0·0047	0·53	0·015	0·05	0·47			Myelin basic protein
MLANA	0·73	0·0027	0·88	0·0070	0·03	0·42	−0·15	0·31	Melan-A
MUC1	4·36	0·024	2·09	0·045	0·02	0·88			Mucin 1, cell surface associated
MYCL1	2·35	0·041	0·94	0·0057	0·14	0·064	0·32	0·090	v-myc myelocytomatosis viral oncogene 1
PLCG1	2·03	0·018	0·86	0·018	−0·04	0·53			Phospholipase C, gamma 1
PRKCA	1·63	0·021	2·40	0·028	0·12	0·067	0·24	0·021	Protein kinase C, alpha
HSP90AA1	0·91	0·0015	−1·14	0·0060	−0·12	0·27			Heat shock protein 90kDa alpha, A1
IGF1R	2·98	0·013	−0·58	0·018	−0·16	0·33			Insulin-like growth factor 1 receptor
RB1	0·73	0·035	−0·67	0·019	−0·06	0·59			Retinoblastoma 1 (including osteosarcoma)
CERK	−0·50	0·040	0·96	0·0035	0·16	0·098	0·02	0·87	Ceramide kinase
HSPD1	−0·66	0·0043	2·49	0·0047					Heat shock 60kDa protein 1 (chaperonin)
TEP1	−1·40	0·20	2·16	0·0009	0·04	0·51			Telomerase-associated protein 1
CYP3A4	−1·07	0·0084	−0·52	0·026	0·03	0·59			Cytochrome P450, 3A4
SOCS3	−0·47	0·0065	−0·83	0·023	−0·27	0·17	−0·56	0·050	Suppressor of cytokine signaling 3
TARP	−0·37	0·0013	1·37	0·013					TCR gamma alt. reading frame protein
TP53	−0·56	0·028	−0·60	0·049	−0·01	0·97			Tumor protein p53

The 17 proteins displaying significant IgG and/or IgM reactivity changes between patients having developed primary graft dysfunction compared with those that did not are listed.

For each protein, the log₂ transformed reactivity ratio and P-value (Student's t-test) for the most significant antigen are shown. Gene expression changes between donor lungs developing PGD compared with those that do not, as measured in two independent studies, are also listed (log₂ transformed expression ratio and P-value from Student's t-test).