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. 2011 Mar;132(3):421–431. doi: 10.1111/j.1365-2567.2010.03382.x

Figure 1.

Figure 1

Interleukin-12 (IL-12) production is suppressed in CD14+ monocyte/macrophage (M/Mφ) during chronic hepatitis C virus (HCV) infection. Peripheral blood mononuclear cells from HCV-infected (▲, n = 25), HCV-resolved (□, n= 15), and healthy subjects (▪, n = 14) were stimulated with lipopolysaccharide (LPS) and R848 ex vivo, followed by cell surface staining for CD14 and intracellular staining for IL-12. The top panel shows the gating strategy for identifying M/Mφ subsets and representative flow cytometric isotype control (left) and dot plot (right) measuring IL-12 production in gated CD14+ M/Mφ with or without LPS/R848 stimulation. The percentage of IL-12-producing cells was calculated in CD14+ cell populations from the study subjects and plotted in the lower panel. Each symbol represents an individual subject, and the horizontal line demonstrates the mean of IL-12+ CD14+ M/Mφ. The P value is shown as *< 0·05, **< 0·01 above the group of study subjects.