Table 1.
The α-gliadin synthetic peptide library used in this study
| Sequences | Peptide | Pool | CXCL111 |
|---|---|---|---|
| Pepsin-trypsin-digested gliadin | 69 | ||
| MVRVPVPQLQPQNPSQQHPQ | 4012 | 2 | 98 |
| PQNPSQQHPQEQVPLVQQQQ | 4013 | 2 | 112 |
| EQVPLVQQQQFLGQQQSFPP | 4014 | 1 | 90 |
| FLGQQQSFPPQQPYPQPQPF | 4015 | 4 | 96 |
| QQPYPQPQPFPSQQPYLQLQ | 4016 | 5 | 103 |
| PSQQPYLQLQPFPQPQLPYL | 4017 | 1 | 94 |
| PFPQPQLPYLQPQPFRPQQP | 4018 | 4 | 93 |
| QPQPFRPQQPYPQPQPQYSQ | 4019 | 5 | 99 |
| YPQPQPQYSQPQQPISQQQQ | 4020 | 1 | 112 |
| PQQPISQQQQQQQQQQQQQQ | 4021 | 4 | 86 |
| QQQQQQQQQQQQILQQILQQ | 4022 | 5 | 55 |
| QLIPCMDVVLQQHNIAHGRS | 4024 | 5 | 116 |
| QQHNIAHGRSQVLQQSTYQL | 4025 | 2 | 106 |
| QVLQQSTYQLLQELCCQHLW | 4026 | 1 | 36 |
| LQELCCQHLWQIPEQSQCQA | 4027 | 3 | 90 |
| QIPEQSQCQAIHNVVHAIIL | 4028 | 3 | 76 |
| IHNVVHAIILHQQQKQQQQP | 4029 | 1 | 93 |
| HQQQKQQQQPSSQVSFQQPL | 4030 | 4 | 126 |
| SSQVSFQQPLQQYPLGQGSF | 4031 | 3 | 100 |
| QQYPLGQGSFRPSQQNPLAQ | 4032 | 3 | 100 |
| RPSQQNPLAQGSVQPQQLPQ | 4033 | 3 | 108 |
| GSVQPQQLPQFEEIRNLALQ | 4034 | 2 | 95 |
| FEEIRNLALQTLPAMCNVYI | 4035 | 5 | 109 |
| TLPAMCNVYIPPYCTIVPFG | 4036 | 2 | 110 |
| PPYCTIVPFGIFGTNYR | 4037 | 4 | 108 |
1
Competitive binding assays performed in our previous study,3 using 125I-labelled CXCL11 and competitors (pepsin-trypsin-digested-gliadin or peptides) on CXCR3-expressing membranes from CHO-K1 cell lines. Data are in % binding of CXCL11 to the cell membranes in the presence of competitors; values below 100% indicate displacement of CXCL11 by its competitors. More details on the procedure are described in Ref. 3.