Skip to main content
. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Nat Med. 2010 Dec 5;17(1):64–70. doi: 10.1038/nm.2266

Figure 3.

Figure 3

Analysis of expression of activation markers, pro- and anti-inflammatory cytokines and markers for M2 macrophages in BMDMs with ectopic overexpression of miR-124. (a,b) Flow cytometry analysis of the expression of CD45 (x axes) and either CD11b, F4/80, MHC class II or CD86 (y axes) in BMDMs transfected with miR-124 or control miRNA. Percentages of CD45hi cells (right quadrants) and activation marker–positive cells (upper quadrants) are shown. A representative experiment is shown in a, and mean ± s.e.m. of four independent experiments is shown in b. MFI, mean fluorescence intensity. *P < 0.05. (c) Flow cytometry analysis of TNF-α production by BMDMs transfected with miR-124 or control miRNA. (d) Real-time qRT-pPCR analysis of the mRNA expression of C/EBP-α, inducible form of nitric oxide synthase (iNOS), IL-4, IL-10, TGF-β1, arginase I and FIZZ1 in BMDMs transfected with miR-124 or control miRNA. (e) Analysis of TGF-β1 protein expression. The cells were stained for intracellular TGF-β1 or for isotype control and analyzed by FACS. Open histograms show TGF-β1 staining; filled histograms show staining for isotype control. Percentages of TGF-β1+ cells are shown.